The pancreatic slate cells are closely related to the formation and metastasis of pancreatic ductal adenocarcinoma, the fibrosis of the pancreas, and the abnormal immunophenotype of pancreatic cancer. Previous studies found that the slate cells were divided into 2 subtypes, resting state and activated state. However, human activated cells were classified into 2 categories by single cell sequencing, namely, immune activated slate cells and standard activated slate cells.

Another kind of interesting cell is the spinal cord. We assume that it is Schwann cells, but the myelin sheath expression in these cells is very low or not. There are some up-regulated expression of stress genes following nerve injury. So we identified this type of cell as a differentiated Schwann cell due to extraction and culture.

3.5 beta cell heterogeneity

By PCA analysis, it is found that different functions of beta cells are not the number of genes, but the expression of the characteristic genes. In conclusion, the genes with low PC1 value are mainly related to the stress function of endoplasmic reticulum, and the genes with high PC1 value are mainly consistent with the function of beta gene. Endoplasmic reticulum stress, that is, the unfolded protein reaction of beta cells, is caused by high levels of insulin synthesis. Due to the high need for insulin secretion, beta cells participate in endoplasmic reticulum stress response.

Comparison of 3.6 single cell sequencing and large number of cell sequencing results

After adjusting the proportion of cells in a large number of cells, it was found that there were no significant differences in the 2369 differential genes found before adjusting the proportion of cells, and 233 genes that had not been identified before were found.

research conclusion

In this study, we constructed the transcript map of human and mouse pancreatic cells, compared them to known cell types, and pointed out their functions, which made us know more about less known cells, such as slate cells.

In addition, through a large number of cells before sequencing identified as differentially expressed genes between diabetic patients and healthy blood donors, may be just because of changes in cell type proportion is caused by the false positives, or because of changes in the proportion of cells and hides some variation, which is false negative. And we can find more precise changes in gene expression through group single cell sequencing, so as to identify the changes of cell microenvironment and the mechanism of occurrence.

膵臓星狀細(xì)胞と膵臓管腺がんによって生成され、転移、すい臓の性質(zhì)、すい臓がんの異常な免疫が密接に関連している。前に研究し発見星狀細(xì)胞は靜止?fàn)顟B(tài)とアクティベーションの亜型に、を通して単細(xì)胞シークエンシング発見アクティベーションの細(xì)胞はまた人に分けに類は、それぞれ、免疫活性化星狀細(xì)胞や基準(zhǔn)星狀細(xì)胞活性化。

また面白いのは神経細(xì)胞の類の脊椎、私たちの仮説は施旺細(xì)胞が、この細(xì)胞の髄鞘表現(xiàn)は低いさえないの。神経が損傷した後のストレス遺伝子が上がっている。だからこれらの細(xì)胞から認(rèn)定抽出と育成による細(xì)胞分化の施旺。

3.5β細(xì)胞の異質(zhì)性

PCA分析を通じて発見して、分けて機(jī)能のβ細(xì)胞の遺伝子の數(shù)はではなく、特徴の表現(xiàn)性遺伝子。まとめて：PC1低値の遺伝子の主要と小胞體のストレス機(jī)能関連、PC1値の高い遺伝子主β遺伝子が機(jī)能と一緻。基本網(wǎng)ストレス、つまりβ細(xì)胞の未折りたたみタンパク反応は高レベルのインスリン合成によるものです。インスリンの分泌が高まっているため、β細(xì)胞はネット上のストレスに反応している。

3 . 6単細(xì)胞シークエンシングと大量細(xì)胞シークエンシング結(jié)果の比較

調(diào)整で大量の細(xì)胞に細(xì)胞の割合が変化した後、にして見つけたに違い、369の遺伝子、調(diào)整は細(xì)胞の割合の変化した後、差別が存在しない顕著性、そして見つけた223の前には異なる遺伝子。

結(jié)論を研究する

本研究を構(gòu)築する人とマウスの膵臓細(xì)胞の転寫本図鑑、それらを照合既知の細(xì)胞のタイプは、それらの機(jī)能がより明確に理解する認(rèn)識の少ない細(xì)胞、例えば星狀細(xì)胞。

また、前に大量の細(xì)胞シークエンシング確定では糖尿病患者と健康獻(xiàn)血者の間の違い表現(xiàn)の遺伝子が、たった細(xì)胞のタイプの割合の変化、つまり偽陽性、あるいは細(xì)胞から割合の変化に隠したいくつかの変異は、偽陰性。私達(dá)によって群體単細(xì)胞シークエンシングより正確遺伝子発現(xiàn)の変化を探して、それによって確定糖尿病発生時の細(xì)胞マイクロ環(huán)境の変化や発生のメカニズム。

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