1.1.3. 培養(yǎng)基 NCCLS推薦使用Mueller-Hinton（MH）肉湯，pH7.2~7.4。需氧菌及兼性厭氧菌在此培養(yǎng)基中生長(zhǎng)良好。在測(cè)試葡萄球菌對(duì)苯唑西林的敏感性時(shí)，應(yīng)在肉湯中加入2%（W/V）氯化鈉，按制造廠家的要求配制需要量的MH肉湯。嗜血桿菌屬菌使用HTM肉湯，肺炎鏈球菌和其它鏈球菌使用含2%~5%溶解馬血的MH肉湯。
1.1.4.接種物的制備有2種方法配制接種物，一是細(xì)菌生長(zhǎng)方法，用接種環(huán)挑取形態(tài)相似待檢菌落3-5個(gè)，接種于4-5ml的水解酪蛋白（MH）肉湯中，35℃孵育2-6h。增菌后的對(duì)數(shù)生長(zhǎng)期菌液用生理鹽水或MH肉湯校正濃度至0.5麥?zhǔn)媳葷針?biāo)準(zhǔn)，約含1~2×108CFU/ml。二是直接菌落懸液配制法，對(duì)某些苛養(yǎng)菌，如流感嗜血桿菌、淋病奈瑟菌和鏈球菌及甲氧西林耐藥的葡萄球菌等菌株，推薦直接取培養(yǎng)18~24h的菌落調(diào)配成0.5麥?zhǔn)媳葷針?biāo)準(zhǔn)的菌懸液。用MH肉湯將上述菌懸液進(jìn)行1∶100稀釋后備用。注意應(yīng)在15分鐘內(nèi)接種完配制好的接種物，并取一份接種物在非選擇性瓊脂平板上傳代培養(yǎng)，以檢查接種物純度。

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1.1.3. Media NCCLS recommended Mueller-Hinton (MH) broth, pH 7.2 ~ 7.4. Aerobes and facultative anaerobes grow well in this medium. In testing for the susceptibility of staphylococci to oxacillin, 2% (w / v) sodium chloride should be added to the broth and the required amount of MH broth prepared as required by the manufacturer. Haemophilus bacteria use HTM broth, Streptococcus pneumoniae and other Streptococcus MH broth containing 2% ~ 5% horse blood.
1.1.4. Preparation of inoculum There are 2 ways to prepare inoculum, one is bacterial growth method, inoculation loop picking colonies with morphology similar to be 3-5, inoculated in 4-5ml hydrolyzed casein (MH) meat Soup, incubated at 35 ℃ 2-6h. Bacteria after enrichment logarithmic growth phase with normal saline or MH broth concentration adjusted to 0.5 McNeil turbid standard, containing about 1 ~ 2 × 108CFU / ml. Second, the direct colony suspension preparation method, for some fastidious bacteria, such as Haemophilus influenzae, Neisseria gonorrhoeae and Streptococcus and methicillin-resistant Staphylococcus aureus and other strains, it is recommended to take direct culture of colon from 18 to 24h Dispense into 0.5 Microbubble turbidity standard bacterial suspension. The above bacteria suspension was diluted 1: 100 with MH broth and set aside. Note that the prepared inoculum should be inoculated within 15 minutes and an inoculum should be subcultured on non-selective agar plates to check for inoculum purity.

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